Ghrelin对绵羊早期体外胚胎发育的影响

doi:10.11843/j.issn.0366-6964.2014.06.010

Abstract

Abstract:

In order to investigate the effects of Ghrelin on the development of early ovine embryos.Early ovine embryos were prepared by in vitro fertilization.The fertilized embryos were cultured in four groups of culture medium:①The group of adding gradiently 300，600，1 000，4 000，10 000 ng•mL^-1 Ghrelin;②The group of adding gradiently 1，10，200，400 ng•mL^-1 Ghrelin receptor antagonist;③The group of containing 400 ng•mL^-1 Ghrelin receptor antagonist and adding gradiently 300，1 000，4 000 ng•mL^-1 Ghrelin;④The control group.Embryo development was observed on the 2nd，3rd，4th，5th day，and the cleavage rates of ovine embryos were calculated at 2-cell，4-cell,8-cell and 16-cell stages.The results showed that: Compared with control group，ovine embryo cleavage rates had no significant change in the group adding Ghrelin (P>0.05)；Embryo cleavage rate had no obvious change at 2-cell，16-cell stages (P>0.05)，but decreased significantly at 4-cell，8-cell stages，and extremely significant difference at 200，400 ng•mL^-1 in the group adding Ghrelin receptor antagonist (P<0.01)；Embryo cleavage rates was recovered slowly on 4-cell，8-cell stages by gradiently adding Ghrelin but still significant difference in the group containing Ghrelin receptor antagonist(P<0.05).These results indicate: Ghrelin promote ovine embryo development at 4-cell and 8-cell stages.

CLC Number:

S826.2

YANG Yan-yan，QING Ge-le，DA Lai,ZHANG Guan-hua,PENG Can-quan,SHAO Kai，SU He，TIAN Ying，WANG Xin-qi，GUO Yan-hua,WU Da-ba-la，ZHOU Xuan，CAO Gui-fang. Effects of Ghrelin on the Development of Early Ovine Embryos in vitro[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(6): 926-931.

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References

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Effects of Ghrelin on the Development of Early Ovine Embryos in vitro

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